Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Canine Carbohydrate Antigen 15-3 (also known as CA15-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine CA15-3 antibody. CA15-3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine CA15-3 Antibody is added and binds to CA15-3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CA15-3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine CA15-3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm